TaqMan and OpenArray Genotyping

More Information

For more information about Taqman and OpenArray Genotyping services, please contact either Hardeep Ranu, Ph.D. (617-432-4469) or Immaculata De Vivo.

For more information about Whole Genome Amplification services, please contact Patrice Soule (617-432-6101).

ABI Prism Taqman Allelic Discrimination Assay

Applied Biosystems's (ABI) Taqman Assay is performed using ABI Prism 7900 HT Sequence Detection System, which allows single-plex SNP interrogation through fluorescence detection in real-time PCR. 

 

Pricing

TaqMan

 

 

  Internal Partners or HSPH External Fee: Non-Partners, AMC and Non-Profit External Fee: For-Profit and Industry DF/HCC Price
Set-Up Fee less than 700 samples  $250 $360 $398 $218
Set-Up Fee 700-2000 samples $400 $576 $636 $349
Set-Up Fee 2000 samples or more $905 $1,303 $1,439 $788
TaqMan Assay genotyping cost per SNP per samples $0.64 $0.92 $1.02 $0.56

 

 

OpenArray SNP Genotyping Platform

The OpenArray SNP Genotyping Platform allows researchers to run 16, 32 or 64 Taqman assays on one OpenArray chip. The OpenArray chips require only 33nl of assay allowing for significant cost savings.

Pricing 

As pricing varies according to the number of samples and number of SNPs that are run, please contact Hardeep Ranu for pricing information. As a guide (for internal Partners or HSPH pricing only) please use this table:

OpenArray Set-Up Fee (per project): $226

 # Samples 16 SNPs 32 SNPs 64 SNPs
<350  $0.51 $0.30 $0.30
<500 $0.27 $0.16 $0.16
<1500 $0.22 $0.20 $0.16
<2500 $0.20 $0.15 $0.14
<3500 $0.19 $0.14 $0.13
<4500 $0.18 $0.13 $0.13
<5000 $0.15 $0.13 $0.13

  

Quality Assurance

For new SNP assays, trial primer and assay design will be carried out and a trial genotyping run performed. Depending on those results, we will either proceed with full-scale genotyping or consider alternative genotyping methods as necessary. Quality control procedures include repeat genotyping of at least 5% of DNA samples. Genotype results are provided to the user in electronic format. Extensive bioinformatics support for our Core Facility is in place.

If you require NHS1/NHS2/HPFS or PHS genotyping data that was not generated using our facility, to be entered in to our Oracle database, we charge a $1448.00 data management fee. 

 

DNA Extraction and Whole Genome Amplification Service 

Whole genome amplification using the GenomiPhi DNA Amplification Kit from GE Inc is used to amplify linear genomic DNA by Multiple Displacement Amplification (MDA). This Kit is based on the high processivity, high fidelity, and strand displacement ability of bacteriophage Phi29 DNA polymerase. The typical yield is 3-7 micrograms of amplified DNA from as little as 10-20 ng of genomic DNA template.

 

 
Pricing

 

  Internal Partners or HSPH External Fee: Non-Partners, AMC and Non-Profit External Fee: For-Profit and Industry DF/HCC Price
DNA Extraction (extract, plate & quantitate DNA) $14.00 $20.16 $22.16 $12.20
Whole Genome Amplification $14.00 $20.16 $22.16 $12.20
Sample Plating Only $1.03 $1.48 $1.64 $0.92
DNA Concentration $1.51 $2.17 $2.40 $1.32
Sample Standardization $3.00 $4.32 $4.77 $2.61

Other sample preparation services are available please contact Pati Soule for more information